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Title: | Detection of Influenza A virus, Respiratory syncytial virus and Human metapneumovirus in guinea pigs (Cavia porcellus) raised as livestock in Paute, Ecuador |
Authors: | García Bereguiain, Miguel Rodríguez Pazmiño, Ángel Sebastián |
Keywords: | Virus de Influenza A Virus sincitial respiratorio Metapneumovirus humano Cavia porcellus Cuyes Infecciones respiratorias Guinea pig Respiratory infections |
Issue Date: | Aug-2019 |
Publisher: | Universidad de Investigación de Tecnología Experimental Yachay |
Abstract: | Los agentes virales tales como Influenza A, virus sincitial respiratorio y metapneumovirus humano son algunos de los más frecuentes patógenos respiratorios humanos y una preocupación para la salud pública mundial. Los cuyes (Cavia porcellus) podrían ser un potencial vector de estos patógenos, debido a su susceptibilidad a ser inoculados por estos microorganismos, lo que ha sido demostrado principalmente en condiciones de laboratorio. Sin embargo, es imperativo hacer diagnósticos de muestras obtenidas de animales criados como ganado, para confirmar o descartar un posible rol del cuy como un vector zoonótico. En países como el Ecuador, donde la producción anual de estos animales es considerablemente alta, este tipo de estudios podría tener una gran relevancia para la salud pública. En la presente investigación, se obtuvieron ochenta muestras de lavado nasofaríngeo de cuyes del cantón Paute (provincia del Azuay). Se realizaron diagnósticos de los virus respiratorios Influenza A, virus sincitial respiratorio y metapneumovirus humano, a través de protocolos conocidos como RT PCR de una etapa y RT PCR de dos etapas. Todas las pruebas produjeron resultados negativos para los tres virus analizados y, por tanto, estos no fueron aislados. |
Description: | Viral agents such as Influenza A virus, Respiratory syncytial virus and Human metapneumovirus are some of the most frequent human respiratory pathogens and a concern for the public health. Guinea pigs (Cavia porcellus) could be a potential reservoir of these pathogens, because their susceptibility to be inoculated by these microorganisms which has been mainly demonstrated in laboratory conditions. However, it is imperative to make diagnoses from samples obtained from animals raised as livestock, to confirm or ruled out a possible role of the guinea pig as a zoonotic vector. In countries such as Ecuador, where the annual production of these animals is considerably high, this type of studies could have greater relevance for public health. In the present investigation, eighty samples of nasopharyngeal washing of guinea pigs from the Paute canton in the province of Azuay were obtained. Diagnoses of the respiratory viruses Influenza A virus, Respiratory syncytial virus and Human metapneumovirus were made, through protocols known as One Step RT PCR and Two Step RT PCR. All the tests yield negative results for the three viruses analyzed and, therefore, the viruses were not isolated. |
URI: | http://repositorio.yachaytech.edu.ec/handle/123456789/102 |
Appears in Collections: | Biología |
Files in This Item:
File | Description | Size | Format | |
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ECBI0013.pdf | 955.36 kB | Adobe PDF | View/Open |
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