Please use this identifier to cite or link to this item: http://repositorio.yachaytech.edu.ec/handle/123456789/206
Title: Preliminary phytochemical characterization of extracts of the orchid Epidendrum coryophorum
Authors: Morera Córdova, Vivian
González Mera, Irina Francesca
Keywords: Orchidaceae
Epidendrum coryophorum
Phytochemical screening
UV-VIS analysis
Total phenolic content
Antimicrobial activity
Anti-inflammatory activity
Orquidaceae
Epidendrum coryophorum
Marcha fitoquímica
Análisis UV-VIS
Contenido fenólico total
Actividad antimicrobiana
Actividad antiinflamatoria
Issue Date: Apr-2020
Publisher: Universidad de Investigación de Tecnología Experimental Yachay
Abstract: Las plantas son de gran importancia para los seres humanos ya que son fuente de alimentos, materiales combustibles, fibras, y sustancias que mitigan el dolor, la fatiga, y alivian los síntomas de muchas enfermedades. Orchidaceae es la segunda familia más grande en el reino de las plantas con flores. En esta familia existen alrededor de 850 géneros y más de 20 000 especies. A lo largo de la historia las orquidáceas, distinguidas por sus flores atractivas y complejas, han sido utilizadas como ornamento, aromatizantes, saborizantes, y alimento, así como en la medicina tradicional y celebraciones religiosas. El objetivo de este trabajo fue realizar el análisis fitoquímico preliminar de extractos de Epidendrum coryophorum. Los especímenes usados en este estudio fueron adquiridos de Ecuagenera (http://www.ecuagenera.com). El tratamiento de las hojas consistió en limpiarlas con agua destilada y luego secarlas a la sombra a una temperatura de 30°C. Posterior a la trituración mecánica del tejido, se realizaron extracciones solido-líquidas para obtener extractos en metanol, etanol y cloroformo. Todos los extractos de hojas se sometieron a un análisis fitoquímico cualitativo. Se detectaron fenoles, flavonoides, cumarinas, taninos, terpenoides, esteroides, esteroles, glucósidos cardiotónicos, carbohidratos, y azúcares reductores. Se determinó el contenido fenólico total de cada extracto mediante el método de Folin- Ciocalteu. Los valores más altos se encontraron en los extractos metanólicos y etanólicos. Adicionalmente se evaluaron la actividad antimicrobiana y la actividad antiinflamatoria. En los extractos metanólicos y etanólicos se obtuvieron resultados positivos.
Description: Plants are of great importance to human beings as they are sources of food, combustible materials, fibers, and substances that mitigate pain, fatigue, and relieve the symptoms of many diseases. Orchidaceae is the second largest family in the kingdom of flowering plants. In this family, there are about 850 genera and more than 20,000 species. Throughout history, orchids, distinguished by their attractive and sophisticated flowers, have been used as ornaments, flavoring, and food, as well as in traditional medicine and religious celebrations. The objective of this work was to perform the preliminary phytochemical analysis of extracts of Epidendrum coryophorum. The specimens used in this study were purchased from Ecuagenera (http://www.ecuagenera.com). The treatment of the leaves consisted of cleaning them with distilled water and then drying them in the shade at a temperature of 30°C. After the mechanical shredding of the tissue, solid-liquid extractions were performed to obtain extracts in methanol, ethanol, and chloroform. All leaves extracts were subjected to a qualitative phytochemical analysis. Phenols, flavonoids, coumarins, tannins, terpenoids, steroids, sterols, cardiotonic glycosides, carbohydrates, and reducing sugars were detected. The total phenolic content of each extract was determined by the Folin-Ciocalteu method. The highest values were found in the methanolic and ethanolic extracts. Additionally, antimicrobial activity and anti-inflammatory activity were evaluated. In the methanolic and ethanolic extracts, positive results were obtained.
URI: http://repositorio.yachaytech.edu.ec/handle/123456789/206
Appears in Collections:Química

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