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http://repositorio.yachaytech.edu.ec/handle/123456789/90| Title: | Experimental evaluation of different compounds like thiadiazine and their anti-malarial effect in the murine model Infected with plasmodium berghei ANKA and plasmodium yoelii 17XL Strains |
| Authors: | Spencer Valero, Lilian Maritza Rodríguez Cabrera, Hortensia María Loachamin Gualotuña, Katherine Stefania |
| Keywords: | Plasmodium berghei Plasmodium yoelii Bis-THTT Anti-malarial drugs Fármacos antipalúdicos |
| Issue Date: | Aug-2019 |
| Publisher: | Universidad de Investigación de Tecnología Experimental Yachay |
| Abstract: | La malaria es una enfermedad potencialmente mortal causada por parásitos protozoarios del género Plasmodium y se transmite a los humanos por la picadura de mosquitos infectados del género Anopheles. En el 2017 la OMS reportó 219 millones de casos de malaria y se estimaron 435,000 muertes. El tratamiento antipalúdico que se usa con mayor frecuencia se basa en la cloroquina, que se ha utilizado durante varias décadas. Esta aplicación prolongada ha provocado que el parásito desarrolle resistencia al uso del medicamento mencionado, por lo que se hace necesario buscar nuevos tratamientos. En el presente trabajo, seis compuestos derivados de bis-THTT (JH1, JH2, JH3, JH4, JH5 y JH6) se evaluaron como posibles fármacos antipalúdicos en ratones BALB/c infectados con las cepas Plasmodium berghei ANKA y Plasmodium yoelii 17XL. Además, evaluamos la respuesta humoral de cada compuesto experimental, mediante una prueba ELISA indirecta, utilizando cada compuesto como antígeno. Finalmente, evaluamos la relación que existe entre la estructura química y la actividad biológica de cada compuesto. Nuestros resultados mostraron que los compuestos JH2 y JH4 presentaban un control eficaz de la parasitemia en ratones infectados con P. berghei. Además, los compuestos JH5 y JH6, exhibieron resultados similares en el control de infección que la cloroquina en ratones infectados con P. yoelii. |
| Description: | Malaria is a life-threatening disease caused by protozoa parasites of the genus Plasmodium and it is transmitted to humans by the bite of infected mosquitoes of the genus Anopheles. WHO has reported 219 million cases of malaria and 435,000 deaths were estimated in 2017. The anti-malarial treatment more frequency used is based on chloroquine, which has been used for several decades. This prolonged application has caused that the parasite developed resistance to the use of mentioned drug, so it becomes necessary to search for new treatments. In the present work, six bis-THTT derivatives compounds (JH1, JH2, JH3, JH4, JH5 and JH6) were evaluated as potential anti-malarial drugs in BALB/c mice infected with Plasmodium berghei ANKA and Plasmodium yoelii 17XL strains. In additional, we evaluated the humoral response of each experimental compounds by indirect ELISA test using each compounds as antigen. Finally, we evaluate the relationship that are between chemical structure and biological activity of each compounds Our results showed that JH2 and JH4 compounds, presented effective parasitaemia control in mice infected with P. berghei. Besides, JH5 and JH6 compounds, exhibited similar results in the infection control as chloroquine in mice infected with P. yoelii. |
| URI: | http://repositorio.yachaytech.edu.ec/handle/123456789/90 |
| Appears in Collections: | Biología |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| ECBI0007.pdf | 2.27 MB | Adobe PDF | View/Open |
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